rabbit anti cd45 fitc  (Bioss)

Journal: iScience

Article Title: Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis.

doi: 10.1016/j.isci.2024.109547

Figure Lengend Snippet: Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

Article Snippet: Antibodies Anti-pan cytokeratin Abcam ab7753 P-Selectin polyclonal antibody Proteintech 13304-1-AP CD45R (RA3-6B2) Santa Cruz sc-19597 Alexa Fluor 488-labeled goat anti-mouse lgG (H + L) Proteintech SA00013-1 594-labeled goat anti-rabbit lgG (H + L) Proteintech SA00013-4 TRITIC-labeled goat anti-Rat lgG (H + L) Proteintech SA00007-7 CD45 Rabbit Polyclonal antibody Proteintech 20103-1-AP FITC anti-mouse CD326 (Ep-CAM) Biolegend 118207 Alex 594 anti-mouse CD45 Biolegend 103144 Chemicals, peptides, and recombinant proteins Fetal bovine serum ExCell Bio FSP500 RPMI-1640 medium HyClone SH30027.02

Techniques: Activation Assay, Control, Staining, Gene Expression, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Enhanced Expression of Glycolytic Enzymes and Succinate Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute Myeloid Leukemia

doi: 10.3390/ijms25042140

Figure Lengend Snippet: Expression of mesothelin in myeloblast subpopulation of AML patients at diagnosis and after induced therapy. ( A ) Gating strategy for AML bone marrow (2 × 10 5 cells) based on CD45 expression. Myeloblast subsets are identified as CD45 dim /SSC-A low . Mature lymphocyte subsets are identified as CD45 high /SSC-A low . Histogram showing mesothelin surface expression in AML bone marrow at diagnosis (red), normal (gray area), and after treatment (blue) in the CD45 dim /SSC-A low myeloblast subsets (Upper). Histogram showing mesothelin expression in AML and normal CD45 high /SSC-A low subsets (Lower). ( B ) Percentage of mesothelin-positive cells in the CD45 dim /SSC-A low myeloblast subsets calculated using Flowjo software (v10.9), represented as a bar graph. ( C ) Percentage of mesothelin-positive cells in the CD45 high /SSC-A low population. ( D ) Mesothelin median fluorescence intensity (MFI) in CD45 dim /SSC-A low myeloblast subsets. Values are presented as mean ± SD (bars) (* p < 0.05, vs. corresponding controls). ( E ) Paired t -test of BF and AF in mesothelin-positive cells percentage. ( F ) Paired t -test of BF and AF in mesothelin MFI (* p < 0.05). HC, Healthy control; BF, Before induced-therapy (IT); AF, after IT.

Article Snippet: A rabbit anti-human mesothelin-APC antibody (Abcam, Cambridge, UK) and rabbit anti-human CD45-FITC antibody (Abcam) were used to analyze cell surface expression of mesothelin in myeloblasts.

Techniques: Expressing, Software, Fluorescence

Journal: Pharmaceutics

Article Title: Hyaluronic Acid Supplement as a Chondrogenic Adjuvant in Promoting the Therapeutic Efficacy of Stem Cell Therapy in Cartilage Healing

doi: 10.3390/pharmaceutics13030432

Figure Lengend Snippet: Surface marker expressions and differentiation potential of rabbit bone-marrow-derived stem/stromal cells (BMSCs). ( A ) BMSCs were separately incubated with each surface marker antibody at 4 °C for 30 min and subjected to flow cytometry analysis. Histogram showing the intensity corresponding to the positive staining of each cell surface marker. ( B ) Histogram showing the intensity of the isotype control. The BMSCs displayed a phenotype profile of CD90 + CD44 + CD73 + CD31 − CD45 − . ( C ) Passage 2 adherent BMSCs cultivated in standard culture medium were found to exhibit a fibroblast-like morphology. ( D ) Alizarin red staining, ( E ) Alcian blue staining, and ( F ) Oil red O staining of BMSCs after osteogenic, chondrogenic, and adipogenic induction for 21 days, respectively.

Article Snippet: Aliquots of 5 × 10 5 cells were incubated with each of the fluorochrome-conjugated antibodies against a panel of cell surface markers, including CD31-FITC (AB9498, Abcam, Cambridge, MA, USA), CD45-FITC (MCA808GA, Bio-Rad, Hercules, CA, USA), CD44-FITC (AB 119335, Abcam, USA), CD73-FITC (AB 175396, Abcam, USA), and CD90-FITC (BD 554895, BD Biosciences, San Jose, CA, USA) at 4 °C.

Techniques: Marker, Derivative Assay, Incubation, Flow Cytometry, Staining, Control